Table 1.
Metabolization pattern and kinetic parameters for CYP71AZs.
| Substrate | Product | Apparent affinity (μM) |
|||
|---|---|---|---|---|---|
| CYP71AZ1 | CYP71AZ3 | CYP71AZ4 | CYP71AZ6 | ||
| Xanthotoxin | 5-hydroxyxanthotoxin | P01: 13.1 ± 3.2 | N.D. | N.D. | P02: 6.3 ± 1.2 |
| Psoralen | Xanthotoxol | N.D. | N.D. | P03: 11.3 ± 2.2 | N.D. |
| 6-Methoxycoumarin | Scopoletin | P11: N.Q. | N.D. | P04: 9.5 ± 1.5 | P12: N.Q. |
| m/z = +16 | N.D. | N.D. | P05: 44.8 ± 16.3 | N.D. | |
| Scopoletin | Fraxetin | N.D. | N.D. | P06: 762.2 ± 586.4 | N.D. |
| 7-Methoxy-3-methylcoumarin | m/z = +16 | N.D. | N.D. | P07: 159.9 ± 41.1 | N.D. |
| 7-Methoxycoumarin | Daphnetin-7-methylether | P09: N.Q. | N.D. | P08: 72.5 ± 15.2 | P10: N.Q. |
| Esculetin | m/z = +16 | N.D. | P13: 248.6 ± 51.9 | N.D. | N.D. |
Reactions were set up in KPi buffer (pH 7.4) at 28°C for 10 min and stopped by the addition of 10 μL of 0.1 M HCl. Products were quantified after HPLC separation at 330 nm. N.D., not detected; N.Q., not quantifiable.