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. 2018 Jun 22;36(27):3926–3936. doi: 10.1016/j.vaccine.2018.05.069

Fig. 2.

Fig. 2

Enzyme-Linked ImmunoSorbent Assays (ELISA). Antisera from individual mice immunised with M2 rT-Nm-MIP-Liposomes and M2 rT-Nm-MIP-Liposomes + MPLA (three independent immunizations each) were reacted against (A) pure M2 rT-Nm-MIP protein, wild-type (WT), (B) MC58 OM, (D) P9-17 OM and (E) FA1090 OM preparations and (C) complemented MC58Δmip::t-nm-mip OM preparation. The columns represent the geometric mean reciprocal ELISA titres (n = 5 animals per group) and the error bars represent the 95% confidence limits. No significant reactivity with pure M2 rT-Nm-MIP protein, WT or complemented OM was observed with sera from sham-immunised animals or with normal mouse serum (NMS) (absorbance values OD450nm < 0.1 for serum dilutions of 1/10). All of the test and control sera showed no significant reactivity (i.e. absorbance values OD450nm < 0.1 for serum dilutions of 1/10) against MC58Δmip, P9-17Δmip or FA1090Δmip OM (data not shown).