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. 2018 Jun 22;36(27):3926–3936. doi: 10.1016/j.vaccine.2018.05.069

Fig. 3.

Fig. 3

Western immunoblotting of wild-type (WT) and mutant meningococcal OM preparations and gonococcal whole lysates. Pooled murine antisera (1/100 dilution; n = 5 animals) raised against purified M2 rT-Nm-MIP-Liposomes and M2 rT-Nm-MIP-Liposomes + MPLA (three independent immunizations each) were reacted against MC58 WT, Δmip and Δmip::t-nm-mip OM preparations (10 µg), or against P9-17 and FA1090 WT and Δmip whole lysates (15 µg), in western blot. MIP protein was recognised as a single band of Mr ∼ 29 kDa in (A) MC58 WT OM preparation and (D) P9-17 and (F) FA1090 WT lysates (identified by the arrow). (C) Truncated Nm-MIP protein was recognised as a single band of Mr ∼ 14 kDa in MC58 complemented OM preparation (identified by the arrow). No significant reactivity was observed with (B) MC58Δmip OM preparation, (E) P9-17Δmip or (G) FA1090Δmip whole lysates with any of the sera tested. All sham immunisation sera and NMS were non-reactive.