Table 1.

Results of transfection experiments. Percentages are calculated on the total number of colonies picked up for each experiment. KO = SURF^−/−; HET = SURF^+/−. *colonies are not targeted (because the HR vector was not used in this transfection), but positive for the introduction of site-specific InDels.

Transfection mix	Fibroblast cell line ID (M/F)	N° colonies	N° targeted colonies (%)	N° sequenced colonies (%)	N° colonies suitable for SCNT (%)
Tal01L + R + HR vector	6639 (M)	48	32 (67%)	14 (29%)	10 (21%) KO
Tal02L + R + HR vector	6639 (M)	42	23 (55%)	8 (19%)	3 (7%) KO
Tal01L + R + HR vector	167 (F)	38	25 (66%)	5 (13%)	5 (13%) HET
CRISPR PAM90	6639 (M)	118	16 (14%)*	79 (67%)	10 (8%) HET
CRISPR PAM90 + HR vector	clone-5	100	14 (14%)	40 (40%)	10 (10%) KO