Fig. 4.

Weakly activated DCs lack long-term metabolic reprogramming. a Gene expression of glucose transporter Glut1 and glycolytic enzymes Hk2, Pkm2, Gapdh, and Ldha by DCs were measured after differential activation for 4 h (left) and 18 h (right). b, c Immunoblot for p-AKT (Ser473), p-AKT (Thr308), p-TBK (Ser172), p-S6K, p-S6, HIF-1α, and β-actin following (b) differential activation for 4 and 18 h, and (c) activation by LPS or HDM over a time course from 1 to 18 h. d Geometric MFI of iNOS in DCs activated by LPS or HDM over a time course from 1 to 18 h. Data shown in a are fold changes from three experiments, b, c one experiment representative of three experiments, and d one experiment representative of two experiments (mean and s.e.m. (a) and s.d. (d) of triplicates). Data were analyzed using t-test, comparing each condition relative to the control. **p < 0.01, *p < 0.05