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. 2018 Jun 25;8:9648. doi: 10.1038/s41598-018-27880-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ventricular zone cells express proliferation markers phosphorylated histone -H3 (pHH3) and proliferating cell nuclear antigen (PCNA). (A) Within the sulcus septomedialis, pHH3+ cells are restricted to the ventricular zone. Expression was confirmed using immunohistochemistry (inset). (B) Likewise, PCNA+ cells are most abundant in the ventricular zone, although occasionally observed in close contact to the ventricular zone within the inner plexiform layer (white arrow). Neither pHH3+ nor PCNA+ cells were ever observed within the cellular layer. Asterisk indicates an artifact. Scale bar A: 10 μm. cl = cellular layer, ipl = inner plexiform layer, opl = outer plexiform layer, vz = ventricular zone.