Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jun 26;8:9679. doi: 10.1038/s41598-018-28143-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

C3a induces overexpression of C3aR in the basolateral membrane of the RPE. (a) Immunolabeling with C3aR antibodies of hfRPE cells treated with different doses of C3a (C3aR-ant) or C3a + C3aR antagonist (C3aR + ant) for 2 weeks. Z-stack was built from images taken every 0.5 µm with confocal microscope. 90° projections show that C3aR is expressed on the basal-lateral membrane of RPE. Scale bars 50 µm. (b) Average quantification of C3aR fluorescent signal of hfRPE cultures treated with different doses of rhC3a (C3aR-ant) or rhC3a + C3aR antagonist (C3aR + ant). (ANOVA, n = 4/C3aR-ant and n = 4/C3aR + ant treatment. Data represented as mean ± SEM. *p < 0.05, **p < 0.01). (c) mRNA levels of C3aR of hfRPE cells treated with different doses of C3a for 24 hours (ANOVA, n = 6. Data presented as mean ± SD. **p < 0.01).