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. 2018 Jun 26;9:2479. doi: 10.1038/s41467-018-04871-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Structural analysis of pri-mir-18a 71-mer RNA. a A structural model of pri-mir-18a 71-mer RNA obtained using the MC-Fold/MC-Sym server. b Experimental and predicted SAXS data for the pri-mir-18a model are shown in black and red, respectively. c NMR analysis of the 71-mer pri-mir-18a RNA. ¹H, ¹⁵N-HSQC of ¹³C, ¹⁵N-labeled pri-mir-18a in the absence (black) and presence (red) of UP1. Imino signals observed indicating stable base-pairing are shown on the secondary structure of pri-mir-18a (magenta boxes). The signal marked with an asterisk has not been assigned. Nucleotides undergoing large perturbations and/or line broadening upon UP1 binding are highlighted with green boxes (left panel) or green letters and arrows (right panel). d 2D-imino NOESY and e H5 correlated HNN experiment of the 17-mer RNA derived from the terminal loop and flanking stem of pri-mir-18a. Base pairs confirmed by these experiments are boxed in blue and red on the secondary structure of the 17-mer RNA. The first G:C base pair, which is not part of the native sequence is shown in lower case. f 2D-imino NOESY of the 17-mer(A35C) RNA. The mutated nucleotide is shown in red. Base pairs confirmed by the detection of the imino correlation are boxed