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. 2018 Jun 18;8:9252. doi: 10.1038/s41598-018-27693-7

Figure 2.

Figure 2

Observation results of fluorescent beads of 3-μm diameter in a transparent epoxy resin tilted at 0.0873 rad (5°). (a) Photograph of the transparent epoxy resin tilted by a goniometric rotation stage. (b) Excitation light intensity. The excitation light intensity was measured under the objective lens. (ce) xz-projected images for an optical depth of 0 μm to 2,000 μm obtained from scans performed with wavefront modulation using ACMSS, with wavefront modulation using SACM, and without wavefront modulation, respectively. (fh) xz image of the observed bead at a 35-μm optical depth when a TPM scan was performed with wavefront modulation using ACMSS, with wavefront modulation using SACM, and without wavefront modulation, respectively. (ik) xz image of the observed bead at a 1,948-μm optical depth. (l) Quantitative evaluation of the improvement in the fluorescence intensity from the fluorescent beads. The scale bars indicate 100 μm in (ce) and 20 μm in(fk).