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. 2018 Jun 18;8:9252. doi: 10.1038/s41598-018-27693-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Observation results of blood vessels in a mouse cerebrum. (a)–(c) $x z$ -projected images for an optical depth of 0 μm to 2,000 μm from scans performed with wavefront modulation using the ACMSS, with wavefront modulation using the SACM, and without wavefront modulation, respectively. (d)–(f) False-colour images with gamma correction and background subtraction of (a–c). (g–i) Magnified $x z$ -projected images from 950 to 1,100 μm. Scale bars indicate 100 μm in (a–c), and 20 μm in (g–i).