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. 2018 Jun 18;8:9252. doi: 10.1038/s41598-018-27693-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Maximum-intensity z-projection images. (a–c) z-projection images from an optical depth of 350 μm to 500 μm from the scan performed with wavefront modulation using ACMSS, with wavefront modulation using SACM, and without wavefront modulation, respectively. (d–f) $z$ - projection images from 750 μm to 900 μm. (g–i), $z$ - projection images from 1,750 μm to 1,900 μm. Scale bar indicates 50 μm. Magnified views of the vessels in the orange dashed box are shown in the lower left of (a–i). The brightness of each image is normalised using the maximum fluorescence intensity of each image after background-noise subtraction.