Reconciliation of functional gene trees (based on amino acid sequences. Top. AmoA; bottom. HaoA) with species tree for comammox Nitrospira, β-AOB, and γ-AOB genomes. The species tree, based on 14 ribosomal proteins, is shown in gray with the gene-trees super-imposed on top in narrower black lines. Arrows and red dots denote transfer and loss events, respectively. The displayed tree is the most parsimonious tree. Dashed arrows indicate an alternative reconciliation scenario