Table 1.

Characteristics of examined genomes

Bin ID or reference	CG24_A	CG24_B	CG24_C	CG24_D	CG24_E	A2_bin	AAU_ MBR1	AAU_ MBR2	Ca N. inopinata	Ca N. nitrificans	Ca N. nitrosa	OLB3	GWW3	GWW4	N. mosco -viensis	Ca N defluvii
Size (bp)	3,555,106	3,236,455	3,000,613	3,392,746	3,477,523	4,111,999	3,525,021	4,296,753	3,295,117	4,117,083	4,422,398	3,754,263	3,501,770	2,981,542	4,589,485	4,317,083
GC Content	55.7	55.3	56.1	57.8	55.8	55.1	55.1	59.1	59.2	56.6	54.8	60.4	55.8	55.9	62.0	59.0
N50 (bp)	137,244	240,795	172,703	66,498	113,824	150,736	30,005	73,098	3,295,117	174,194	727,365	121,772	184,662	32,587	4,589,485	4,317,083
Number of scaffolds	58	36	28	135	63	61	225	172	1	36	15	79	38	167	1	1
Number of coding sequences	3697	3437	3044	3388	3628	4316	3607	4129	3308	4162	4380	3734	3433	3077	4572	4132
Number of tRNAs	42	35	44	39	36	42	39	49	49	46	49	40	43	37	50	49
Completeness (107 HMM)	95	95	97	97	100	93	90	99	100	100	100	94	98	94	100	100
Redundancy (107 HMM)	2.9	2.0	0.0	1.0	0.9	1.0	2.1	1.0	2.8	0.9	0.9	2.0	1.0	1.0	2.8	0.9
Completeness (CheckM)	88	92	92	92	94	88	86	97	97	97	97	91	92	84	96	98
Redundancy (CheckM)	5.1	6.9	2.7	2.7	3.2	2.8	2.7	5.5	4.8	2.7	2.3	4.3	3.2	1.8	6.6	2.3
Comammox affiliation	clade B	clade A	clade B	No	clade B	clade A	clade A	No	clade A	clade A	clade A	No	clade B	clade B	No	No
Nitrospira Lineage	II	II	II	II	II	II	II	I	II	II	II	I	II	II	II	I
Reference	This study	This study	This study	This study	This study	[14]	[1]	[1]	[1]	[2]	[2]	[82]	[1]	[1]	[11]	[51]

*Minimum sequence length in which 50% of the assembled bases occur