Table 2.
Ex vivo biodistribution of 18F-FOL in mice and rabbits.
| Tissue | LDLR−/−ApoB100/100 atherosclerotic mice (n = 9) | LDLR−/−ApoB100/100 mice + blocking (n = 3) | C57BL/6N control mice (n = 9) | Watanabe atherosclerotic rabbits (n = 4) |
|---|---|---|---|---|
| Aorta | 2.4 ± 0.56 | 0.28 ± 0.15 (P = 0.0040)* |
1.3 ± 0.46 (P = 0.00062)† |
0.075 ± 0.026§ 0.048 ± 0.018║ |
| BAT | 0.89 ± 0.43 | 0.10 ± 0.024 (P = 0.012)* |
0.50 ± 0.37 (P = 0.084)† |
ND |
| Blood | 0.48 ± 0.20 | 0.14 ± 0.059 (P = 0.0037)* |
0.20 ± 0.065 (P = 0.0010)† |
0.0056 ± 0.0027 |
| Heart | 0.66 ± 0.17 | 0.080 ± 0.032 (P < 0.0001)* |
0.46 ± 0.089 (P = 0.0068)† |
0.095 ± 0.086 |
| Kidneys | 110 ± 28 | 9.3 ± 0.63 (P < 0.0001)* |
104 ± 18 (P = 0.77)† |
0.92 ± 0.34 |
| Lungs | 1.2 ± 0.37 | 0.19 ± 0.063 (P < 0.0001)* |
0.54 ± 0.15 (P = 0.00023)† |
0.032 ± 0.012 |
| Liver | 2.8 ± 1.3 | 0.98 ± 0.45 (P = 0.052)* |
1.4 ± 1.1 (P = 0.031)† |
0.55 ± 0.69 |
| Muscle | 0.52 ± 0.10 | 0.059 ± 0.012 (P = 0.0014)* |
0.43 ± 0.23 (P = 0.44)† |
0.015 ± 0.0041 |
| Pancreas | 1.2 ± 0.38 | 0.15 ± 0.037 (P < 0.0001)* |
0.64 ± 0.21 (P = 0.0014)† |
0.15 ± 0.032 |
| Plasma | 0.70 ± 0.18 | 0.20 ± 0.10 (P < 0.0001)* |
0.30 ± 0.11 (P < 0.0001)† |
0.14 ± 0.16 |
| Small intestine | 4.2 ± 2.8 | 4.6 ± 0.85 (P = 0.99)* |
3.1 ± 1.8 (P = 0.70)† |
0.20 ± 0.066 |
| Spleen | 0.34 ± 0.072 | 0.13 ± 0.043 (P = 0.079)* |
0.29 ± 0.20 (P = 0.74)† |
0.75 ± 0.53 |
| Thymus | 1.7 ± 1.0 | 0.20 ± 0.11 (P = 0.012)* |
0.67 ± 0.28 (P = 0.013)† |
ND |
| Urine | 66 ± 48 | 261 ± 97 (P = 0.0013)* |
84 ± 51 (P = 0.50)† |
0.15 ± 0.20 |
| WAT | 0.46 ± 0.16 | 0.058 ± 0.029 (P = 0.0020)* |
0.38 ± 0.16 (P = 0.49)† |
0.027 ± 0.023 |
| Aorta-to-blood | 5.2 ± 1.5 (P < 0.0001)‡ |
2.5 ± 1.8 (P = 0.36)‡ |
6.8 ± 2.6 (P < 0.0001)‡ |
16 ± 10§ (P = 0.013)‡ |
| Aorta-to-heart | 3.6 ± 0.76 (P < 0.0001)‡ |
2.5 ± 1.8 (P = 0.11)‡ |
2.9 ± 0.74 (P < 0.0001)‡ |
1.3 ± 1.0§ (P = 0.67)‡ |
| Aorta-to-muscle | 2.3 ± 0.52 (P < 0.0001)‡ |
0.28 ± 0.059 (P = 0.13)‡ |
1.3 ± 0.42 (P = 0.00015)‡ |
5.6 ± 2.7§ (P = 0.21)‡ |
Results are expressed as a percentage of injected radioactivity dose per gram of tissue (mean ± SD with two significant figures) and obtained at 120 min post-injection. The blocking study was performed by injecting a 100-fold molar excess of folate glucosamine 1 min previous to the 18F-FOL injection.
*Difference between LDLR−/−ApoB100/100 atherosclerotic mice vs. atherosclerotic mice + blocking, as assessed by independent samples t-test.
†Difference between LDLR−/−ApoB100/100 atherosclerotic vs. C57BL/6N control mice as assessed by independent samples t-test.
‡Intra-animal comparison assessed by paired t-test.
§Aorta with advanced atherosclerosis.
║Aorta with mild atherosclerosis.
BAT = brown adipose tissue; WAT = white adipose tissue; ND = not determined.