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ChIP assays of active chromatin markers H3K4me1, H3K27Ac, and H3K4me3 in the ~32-kb LD block as shown in Supplemental Fig. 4. Two fresh frozen thyroid tissue samples were used for the ChIP assays. The relative abundance of the three markers was normalized to immunoglobulin G control. H3K4me1 and H3K27Ac showed significant binding in all the tested regions, with P < 0.005, Student t test.
