Fig. 2.

CRISPR–Cas9-mediated ltd gene disruption in C. reinhardtii. (A) Alignment of the DNA sequences of the wild-type and the Crltd mutants at the ltd locus. All mutants induced by CRISPR–Cas9 had small insertions and deletions (indels) in the ltd gene, which disrupted the start codon. The ATG start codon of the wild-type is indicated by the red box. (B) Coomassie-stained SDS-PAGE and western blot analysis with CrLTD-specific antibodies revealed that CRISPR–Cas9-induced mutations resulted in a deletion of the LTD protein in Crltd strains. Protein loading: 10 µg per lane. (This figure is available in color at JXB online.)