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. 2018 Jun 26;8:9678. doi: 10.1038/s41598-018-27757-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Proportion and presence of assigned CSTs change depending on the 16S region used for amplification. The two regions reveal different CSTs. The proportion of Lactobacillus-dominated and diverse CST appears lower while CSTs characterized by the presence of Gardnerella vaginalis are prominent when using the V3/V4 region in comparison to the V1/V2 data set (Pearson’s chi square test: p = 0.002). The CSTs are named according to characteristic species of each cluster: Lin: Lactobacillus iners, Lcr: L. crispatus, Lga: L. gasseri, div: diverse, Ach: Aerococcus christensenii, Sag: Staphylococcus agalactiae, Gva: Gardnerella vaginalis, Bbi: Bifidobacterium bifidum.