Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jun 26;8:9685. doi: 10.1038/s41598-018-27892-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Assessment of the functional significance of NHEJ repair activity by selective pharmacological inhibition. (a) NU7441 (50 μM) a potent and selective antagonist of DNA-PKcs and (c) SCR7 (20 μM), a selective inhibitor of DNA ligase IV were incubated with ETP-treated oocytes during a recovery time course (6 h). In both instances, inhibition of DNA-PKcs or DNA ligase IV completely prohibited the resolution of γH2A.X foci. In contrast, ETP-treated oocytes not challenged with these pharmacological inhibitors experienced a significant decrease in γH2A.X labelling following an equivalent recovery period. (b and d) Representative immunofluorescence images of γH2A.X labelling of the metaphase plate are presented, with the main image corresponding to oocytes treated with (b) ETP + NU7441 and (d) ETP + SCR7. Similarly, the insets in panels (b) and (d) correspond to γH2A.X labelling of the metaphase plate in ETP treated oocytes at T = 0 (i.e. upper insets) and again at T = 6 h (lower insets). Statistical significance was determined using ANOVA and Student’s t-tests. *p ≤ 0.05, **p ≤ 0.01 and ***p ≤ 0.001. Each experiment was conducted on a minimum of three biological replicates, with each replicate comprising a minimum of 30 oocytes. All data are expressed as means ± S.E.M. Scale bar = 20 μm.