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. 2018 Jun 26;8:9698. doi: 10.1038/s41598-018-28039-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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2D Projection maps of purified dimeric complex III from E. gracilis obtained by single particle averaging. The dimeric complex III was purified by a two-step chromatographic procedure in the presence of β-dodecyl-n-maltoside and analyzed by EM. Overlap of the chicken dimeric complex III (pdb: 4U3F⁶³) over the EM images was performed. (A–D) side view projections, (E–H) slightly tilted side-view. The membrane region is indicated by the green arrow heads. At the right: schematic representation of the rotation of the complex in the left panels. The approximate rotation angles over the axis are indicated. The scale bar is 10 nm.