Table 1.
Euglena Complex I subunits identified by mass spectrometry.
| I | Name | Accession number | MW | MW (calc) | TMH (putative) | Conserved Domain |
|---|---|---|---|---|---|---|
| 1 | unknown | comp60085_c0_seq. 3 | 58.5 | 44.2 | 0 | —a,b |
| 2 | unknown | comp54683_c0_seq. 1 | 58.5 | 18.1 | 0 | —a,b |
| 3 | NDTB5 | comp54702_c0_seq. 3 | 52.2 | 54.9 | 1 (C) | NDUFA9_like_SDR_a (M)a,b |
| 4 | NDTB12 | >gnl|Egra|Contig2023_c | 51.0 | 51.8 | 0 | —a,b |
| 5 | NDUFV1 | comp62912_c0_seq. 6 | 51.0 | 57.8 | 0 | NADH dehydrogenase I subunit F (A)a,b |
| 6 | CAG1 | comp62122_c0_seq. 3 | 48.5 | 48.7 | 0 | LbH_gamma_CA_like (M)a,b |
| 7 | NDTB17 | comp63840_c0_seq. 1 | 48.5 | 53.3 | 0 | Adenylate forming domain, Class I (C)a,b |
| 8 | NDUFA6 | comp61975_c0_seq. 5 | 45.7 | 48.7 | 0 | —a,b |
| 9 | unknown | comp46408_c0_seq. 1 | 45.7 | 8.5 | 0 | —a,b |
| 10 | NDTB2 | comp63125_c0_seq. 1 | 44.8 | 46.4 | 0 | 2-enoyl thioester reductase (N,M)a,b |
| 11 | NDUFS2 | comp55715_c0_seq. 3_c | 41.9 | 48.6 | 1 (M) | NADH dehydrogenase subunit D; NADH:ubiquinone oxidoreductase 49 kD subunit 7 (A)a,b |
| 12 | DnaJ | comp60945_c0_seq. 5_c | 40.0 | 43.6 | 1 (C) | DnaJ molecular chaperone homology domain(N)a,b |
| 13 | NDUFS1 | comp61469_c0_seq. 1_c | 40.0 | 43.8 | 0 | NADH dehydrogenase/NADH:ubiquinone oxidoreductase 75 kD subunit (chain G) (A)a,b |
| 14 | NDUFA9 | comp59654_c0_seq. 4 | 38.7 | 41.3 | 0 | NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, subunit 9, 39 kDa (A)a,b |
| 15 | NDUFA12 | gnl|Egra|Contig149 | 35.7 | 23.2 | 0 | NADH ubiquinone oxidoreductase subunit NDUFA12a,b |
| 16 | ADP/ATP | comp62013_c0_seq. 2_cut_gi|109788168 | 32.4 | 28.3 | 2(C) | Mitochondrial carrier protein x 2(N,M)a,b |
| 17 | GapC3 | comp52123_c0_seq. 2_gi|125990644 | 31.0 | 37.1 | 0 | Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain (C)a,b |
| 18 | unknown | comp58177_c0_seq. 3 | 31.0 | 32.6 | 3(M) | —a,b |
| 19 | NDUFS3 | comp62960_c0_seq. 11 | 31.0 | 32.4 | 0 | NADH dehydrogenase subunit C (M)a,b |
| 20 | CAG2 | comp48089_c0_seq. 3_c | 31.0 | 28.8 | 0 | Gamma carbonic anhydrase-like (M)a,b |
| 21 | NI | — | 27.7 | — | — | — |
| 22 | NI | — | 26.1 | — | — | — |
| 23 | unknown | comp47716_c0_seq. 2_c | 25.5 | 24.0 | 0 | —a,b |
| 24 | unknown | comp51732_c0_seq. 5_ gi|125990596 | 25.1 | 22.6 | 0 | —a,b |
| 25 | NDUFA13 | comp59406_c0_seq. 3 | 25.1 | 22.7 | 1 (M) | GRIM-19 protein (M)a,b |
| 26 | unknown | comp52747_c0_seq. 1_c | 22.9 | 22.0 | 2 (M) | —a,b |
| 27 | NDUFS8 | comp54309_c0_seq. 4_c | 22.4 | 24.3 | 0 | NADH dehydrogenase subunit I (M)a,b |
| 28 | NDUFA5 | comp49716_c0_seq. 2 | 22.4 | 20.8 | 0 | ETC_C1_NDUFA5 (M)a,b |
| 29 | unknown | comp49825_c0_seq. 3_c | 21.3 | 19.5 | 1 (C) | —a,b |
| 30 | p18 | comp56597_c0_seq. 3 | 21.3 | 21.0 | 0 | —a,b |
| 31 | unknown | comp53986_c0_seq. 1_c | 20.9 | 18.3 | 0 | —a,b |
| 32 | NDUFS6 | comp55416_c0_seq. 4 | 20.9 | 17.1 | 0 | —a,b |
| 33 | unknown | comp41364_c0_seq. 3 | 18.8 | 17.7 | 1 (C) | —a,b |
| 34 | NDUFB10 | comp54566_c0_seq. 2 | 18.8 | 17.4 | 0 | —a,b |
| 35 | NI | — | 18.3 | |||
| 36 | unknown | comp54442_c0_seq. 1 | 17.5 | 17.2 | 1 (M) | —a,b |
| 37 | unknown | comp54200_c0_seq. 1 | 17.1 | 15.6 | 1 (N) | —a,b |
| 38 | NI | — | 16.8 | |||
| 39 | unknown | comp51611_c0_seq. 1_c | 16.3 | 14.9 | 1 (M) | —a,b |
| 40 | unknown | comp51117_c0_seq. 1 | 16.3 | 9.9 | 0 | —a,b |
| 41 | NI | — | 15.5 | — | — | — |
| 42 | NI | — | 11.7 | — | — | — |
| 43 | NI | — | 10.7 | — | — | — |
| 44 | NI | — | 8.7 | — | — | — |
| 45 | NI | — | 7.6 | — | — | — |
| Total | 1333 | 1097 |
NI not identified.
When accesion number ends with _c, the first Methionine was used for sequence analysis.
(N) Amino terminus, (C) Carboxi terminus, (M) Middle region, (A) Almost all sequence.
aCCD.
bDELTA-BLAST.
cThe e-value threshold for the blast results was 10−5.