Table 2.
Euglena Complex III subunits identified by mass spectrometry.
| III | Name | Accession number | MW | MW (calc) | TMH (putative) | Conserved Domainc |
|---|---|---|---|---|---|---|
| 1 | QCR1 | comp63646_c0_seq. 8_c | 50.9 | 53.5 | 0 | Peptidase_M16 (N), Peptidase_M16C (C)a,b |
| 2 | QCR2/TB1 | comp60854_c0_seq. 1_c | 47.9 | 51.1 | 0 | Insulinase (Peptidase family M16) (N)a,b |
| 3 | CYT1 | comp49373_c0_seq. 3_c | 31.3 | 28.0 | 1 (C) | Cytochrome C1 family (A)a,b |
| 4 | COB | ALQ28773.1 | 31.3 | 43.9 | 9(A) | cytochrome b (A)a,b |
| 5 | QCR7 | comp51517_c0_seq. 3_c | 30.3 | 27.4 | 1 (N) | Ubiquinol-cytochrome C reductase complex 14kD subunit (C)a,b |
| 6 | RIP1 | comp57996_c0_seq. 3_c | 29.5 | 27.8 | 1 (M) | Rieske_cytochrome_bc1 (C)a,b |
| 7 | unknown | comp47102_c0_seq. 2_c | 22.4 | 20.3 | 1 (C) | —a,b |
| 8 | unknown | comp57617_c0_seq. 1_c | 18.8 | 17.6 | 0 | —a,b |
| 9 | NI | — | 14.7 | — | — | — |
| 10 | NI | — | 10.5 | — | — | — |
| Total | 288 | 270 |
NI not identified.
When accesion number ends with _c, the first Methionine was used for sequence analysis.
(N) Amino terminus, (C) Carboxi terminus, (M) Middle region, (A) Almost all sequence.
aCCD.
bDELTA-BLAST.
cThe e-value threshold for the blast results was 10−5.