Table 3.
Euglena Complex IV subunits identified by mass spectrometry.
| IV | Name | Accession number | MW | MW (calc) | TMH (putative) | Conserved Domain |
|---|---|---|---|---|---|---|
| 1 | COX1 | comp60817_c0_seq. 2_c | 38.5 | 45.5 | 10(A) | Cytochrome C oxidase subunit I (A)a,b |
| 2 | COXTB4 | comp55436_c0_seq. 3_c | 36.4 | 35.6 | 0 | —a,b |
| 3 | COX6B | comp54364_c0_seq. 4 | 35.1 | 33.0 | 0 | —a,b |
| 4 | unknown | comp57506_c0_seq. 2_c | 32.6 | 31.7 | 0 | —a,b |
| 5 | COXTB5 | gi|109781798_c | 25.9 | 24.5 | 0 | —a,b |
| 6 | COX3 | comp54737_c0_seq. 2 | 22.9 | 27.3 | 3 (M, C) | Heme-copper oxidase subunit III (C)a,b |
| 7 | unknown | comp53543_c0_seq. 2 | 22.9 | 20.4 | 1 (M) | —a,b |
| 8 | unknown | comp51338_c0_seq. 1_c | 22.9 | 19.8 | 0 | —a,b |
| 9 | unknown | comp47102_c0_seq. 2_c | 22.0 | 20.3 | 1 (C) | —a,b |
| 10 | COXTB2 | comp54722_c0_seq. 3_c | 22.0 | 19.7 | 0 | —a,b |
| 11 | unknown | comp55710_c0_seq. 1_c | 20.9 | 19.9 | 1 (M) | —a,b |
| 12 | NI | — | 16.7 | — | — | — |
| 13 | unknown | comp50120_c1_seq. 1_c | 14.9 | 16.4 | 1 (N) | —a,b |
| 14 | unknown | comp48005_c0_seq. 1_c | 13.6 | 13.1 | 1 (M) | —a,b |
| 15 | unknown | comp53374_c0_seq. 4_c | 10.6 | 9.8 | 0 | —a,b |
| 16 | NI | — | 7.2 | — | — | — |
| Total | 365 | 337 |
NI not identified.
When accesion number ends with _c, the first Methionine was used for sequence analysis.
(N) Amino terminus, (C) Carboxi terminus, (M) Middle region, (A) Almost all sequence.
aCCD.
bDELTA-BLAST.
cThe e-value threshold for the blast results was 10−5.