Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jun 26;8:9710. doi: 10.1038/s41598-018-27732-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Location of mucins with antibody staining. (a) Left: Surface and foveolar epithelial cells stained brown with an antibody recognizing MUC5AC. Right: gland cells stained brown with an antibody against MUC6. The sections were counterstained with heamatoxylin, outlining the tissue in blue, with the dark blue representing nuclei. (b) Binding of J99 WT and its isogenic adhesin deletion mutant J99ΔbabAΔsabA to mucins from five patients with varying glycosylation and health status (outlined in Table 1) and to pig gastric mucin (PGM). The *** indicates p < 0.001 difference in binding between J99 WT and J99ΔbabAΔsabA to a mucin (Two way ANOVA). The results are shown after subtracting the background signal for the assay in the absence of mucins. The level of binding was relatively weak in comparison to what we previously have showed for some other human mucins¹⁸, although the positive control human mucin (HM6N in the graph) displayed high binding to H. pylori, demonstrating that the low binding is not due to technical issues, but due to the properties of the mucins investigated in this study.