Table 1. Summary of kinetic data.
Ribozyme | Metal ion | 1 mM |
|
|
|
k′ (ep′); (min–1) (%) |
k″ (ep″); (min–1) (%) |
cis(W) | Mg | 3.95 ± 0.75 (15) | 0.14 ± 0.02 (23) |
Ca | 2.16 ± 0.25 (29) | 0.08 ± 0.02 (36) | |
Mn | 8.8 ± 3.9 (11) | 0.18 ± 0.02 (40) | |
cis(L) | Mg | 7.65 ± 0.92 (40) | 0.44 ± 0.05 (36) |
Ca | 5.61 ± 0.69 (45) | 0.48 ± 0.07 (36) | |
|
Mn |
10.36 ± 1.61 (41) |
0.49 ± 0.05 (44) |
kobs (EP); (min–1) (%) |
|
||
|
|
1 mM |
10 mM |
trans(S1) | Mg | 0.19 ± 0.01 (92) | 0.92 ± 0.07 (87) |
Ca | 0.26 ± 0.02 (92) | ||
Mn | 0.24 ± 0.02 (92) | ||
trans(S2) | Mg | 0.17 ± 0.01 (81) | 1.32 ± 0.16 (86) |
Ca | 0.20 ± 0.02 (90) | 2.31 ± 0.32 (86) | |
Mn | 0.57 ± 0.04 (86) | 1.87 ± 0.25 (86) |