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. 2017 Apr 27;28(6):1991–2006. doi: 10.1093/cercor/bhx105

Figure 3.

Figure 3.

CP-AMPAR activation is involved in upregulation of Ngf and Fgf10 mRNA following mGluR5 deletion. Primary cortical neurons derived from global mGluR5 KO mice were treated with either NBQX (AMPAR antagonist, 10 μM), Naspm (CP-AMPAR inhibitor, 10 μM), EGTA (extracellular calcium chelator, 1 mM), or D-APV (NMDAR antagonist, 50 μM) for 8 h. The mRNA levels of Ngf (A), Fgf7 (B), Fgf10 (C), and Fgf22 (D) were measured using real-time qPCR (n ≥ 5). One-Way ANOVA with post hoc Tukey’s multiple comparisons test was used to assess statistical significance. The statistical analysis (*) refers to wild-type neurons compared with mGluR5 KO neurons. ***P < 0.001. (#) refers to the indicated-group compared with ctrl-mGluR5 KO neurons. #P < 0.05; ###P < 0.0001.