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. 2018 Jun 22;13:3593–3608. doi: 10.2147/IJN.S160828

Figure 3.

Figure 3

SiO2-NP treatment induced inflammatory response in the lungs of the 8-day-old mice without alteration in the body and brain weight.

Notes: (A) The effects of SiO2-NP exposure on the average body weights of mice (n=10 per group). (B) The effects of SiO2-NP exposure on brain weight at P8 (n=8 per group). The data are presented as the mean ± SEM. (C–F) The structure of the lung as illustrated by HE staining for the groups: Vehicle, SiO2-NPs (10 mg), SiO2-NPs (20 mg), and SiO2-NPs (50 mg). (G–J) The images are higher-power views of C–F. (K–N) The locations of the SiO2-NPs labeled by FITC as revealed by immunofluorescent staining in the four groups: Vehicle, SiO2-NPs (10 mg), SiO2-NPs (20 mg), and SiO2-NPs (50 mg). (O–R) The images are higher-power views of K–N. Arrows highlight the FITC-labeled SiO2-NP burden observed in alveolar macrophages. The scale bar in F =100 µm and applies to C–F; in J the scale bar =50 µm and applies to G–J; in N the scale bar =25 µm and applies to K–N; and in R the scale bar =10 µm and applies to O–R.

Abbreviations: SiO2-NPs, silica nanoparticles; FITC, fluorescein isothiocyanate; SEM, standard error of the mean; DAPI, 4′,6-diamidino-2-phenylindole.