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Expression of MgaPps1 in E.coli. Proteins contained in soluble crude extracts of (lane 1) non-transformed BL21(DE3)[pLysS], (lane 2) untagged MgaPps1 and (lane 3) tagged MgaPps1 were separated by 10% SDS–PAGE. Proteins were detected either by (A) Coomasie blue staining or (B) western blotting using anti-V5 antibodies.
