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. 2001 Nov 1;29(21):4310–4318. doi: 10.1093/nar/29.21.4310

Figure 4.

Figure 4

Cleavage assay for MgaPps1. Aliquots of 100 ng ScaI-linearized pMgaSite substrate (A) or linearized pUC19 (B) were incubated with 250 ng of a crude extract of (lane 1) non-transformed BL21(DE3)[pLysS], (lane 2) untagged MgaPps1 or (lane 3) tagged MgaPps1, or with (lane 4) 100, (lane 5) 50 or (lane 6) 25 ng of a crude extract of untagged MgaPps1 for 10 min at 37°C, or with 25 ng of a crude extract of untagged MgaPps1 for (lanes 7–12) 0, 10, 30, 40, 100 or 150 min, in 10 mM Tris–HCl, pH 8, buffer containing 10 mM MgCl2 and 25 mM KCl.