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. 2017 Dec 30;69(5):1051–1064. doi: 10.1093/jxb/erx458

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© The Author(s) 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2. — Interaction of OsSEC3A with other exocyst subunits. (A) Y2H assay in S. cerevisiae. Full-length OsSEC3A was ligated into pGBKT7 to generate the OsSEC3A-BD plasmid. Several exocyst constructs were fused with the activation doamin (AD), displaying no auto-activation. (B) In vitro pull-down assay of recombinant GST–OsExo70A1, GST–OsExo70B1, and GST using resins containing MBP–OsSEC3A. Asterisks indicate GST–OsExo70A1 and GST–OsExo70B1. (C) Quantification of luciferase activity in N. benthamiana leaves expressing: OsSEC3A_{_C} (amino acids 521–888)-NLuc and CLuc-OsSEC5; OsSEC3A_{_C}-NLuc and CLuc-OsSEC10; OsSEC3A_{_C}-NLuc and CLuc-OsSEC6; or OsSEC3A_{_C}-NLuc and CLuc-OsSEC15BN. The data shown are representative of three independent experiments.