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. 2017 Dec 28;8(1):2045893217753156. doi: 10.1177/2045893217753156

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© The Author(s) 2018

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Fig. 1. — PP5C is required for the FKBP51-mediated inhibition of I_soc in HEK293 cells. (a) Western blot analysis and (b) densitometry (calculated as band intensity of FKBP51 relative to that of β-actin and normalized to one for control HEK293 cells) analysis reveal that lentiviral transduction of hFKBP51 resulted in acute FKBP51 overexpression in control HEK293 cells (n = 3). (c) Thapsigargin-induced I_soc was recorded using whole-cell patch clamp electrophysiology. Overexpression of FKBP51 (red) resulted in significantly decreased I_soc compared to control HEK293 cells (black) at the indicated testing potentials. *P < 0.05 control vs. FKBP51 overexpressing HEK293 cells; n = 6. (d) Western blot analysis and (e) densitometry (calculated as band intensity of the indicated protein relative to that of β-actin and normalized to one for control HEK293 cells) confirm the disruption of PP5C disruption (PP5C^−/−) utilizing CRISPR/Cas9. Lentiviral transduction of hFKBP51 resulted in overexpression of FKBP51 in PP5C^−/− cells, and in PP5C^−/− cells in which PP5C was reintroduced. *P < 0.05 vs. control; n = 3. (f) Overexpression of FKBP51 did not inhibit I_soc in PP5C^−/− cells (green). Reintroduction of PP5C into cells restored the FKBP51-mediated inhibition of I_soc (gray). Data for control (black) and FKBP51 overexpressing cells (red) are those shown in (c) and are included here for comparison. (g) Catalytic-deficient PP5C was reintroduced into the PP5C^−/− cells (PP5C-ΔCat). An acute overexpression of hFKBP51 was achieved by lentiviral transduction of the PP5C-ΔCat HEK293 cells as shown by western blot and (h) by densitometry (calculated as band intensity of the indicated protein relative to that of β-actin and normalized to one for control PP5C-ΔCat HEK293 cells). *P < 0.05 vs. CatΔ; n = 3. (i) Overexpression of FKBP51 in PP5C-ΔCat cells (red) did not inhibit I_soc relative to control PP5C-ΔCat HEK293 cells (black) (n = 6).