Fig. 11.

Proline accumulation of wild-type and transgenic plants in response to salt and mannitol stresses. (A) The 7-d-old seedlings were transferred to 1/2-MS medium with or without sucrose and grown for 72 h under normal conditions. (B) The 7-d-old seedlings were transferred to 1/2-MS medium supplemented with NaCl (150 mM) with or without sucrose and grown for 72 h. (C) The 7-d-old seedlings were transferred to 1/2-MS medium supplemented with mannitol (300 mM) with or without sucrose and grown for 72 h. Data are means (±SD) of three independent experiments. Significant differences between the wild-type and transgenic plants were determined using Student’s t-test (*P<0.05, **P<0.01).