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. 2018 Jan 31;69(8):2005–2021. doi: 10.1093/jxb/ery035

Fig. 12.

Fig. 12.

Transient expression of LlHsfA3A and LlHsfA3B in lily reduces proline accumulation under salt stress. (A) Flower buds and discs taken from the inner petal (see Methods). (B) Expression levels of LlHsfA3A, LlHsfA3B, and LlproDH2 in overexpressed petals relative to the control (set as 1) as determined by qPCR. Data are means (±SD) of three independent experiments. Significant differences compared with the control were determined using Student’s t-test (*P<0.05). (C, D) Proline content of discs determined after the treatments described in the Methods. (E, F) Relative ion leakage (%) of discs determined after the treatments described in the Methods. Data are means (±SD) of three independent experiments. Each biological replicate included 20 discs. The percentages shown in (C) indicate the within-pair relative increase. Different letters indicate significant differences as determined using Student–Newman–Keuls test (P<0.05).