Fig. 3.

Expression patterns of LlHsfA3A and LlHsfA3B genes in lily leaves under different abiotic stress treatments. Two cultivars, ‘White heaven’ and ‘Siberia’, were investigated. (A, B) Leaf samples were collected after 3 h under different ambient temperature treatments. (C, D) Leaf samples were collected after different periods of exposure to 37 °C. (E, F) Tissue-cultured seedlings of lily with roots were treated with water (control, CK), salt solution (NaCl, 200 mM), or mannitol solution (400 mM) for 24 h, after which their leaves were collected. The data were normalized to 18S rRNA of lily, and the 2^–ΔΔCt method was used in the qPCR analysis. Each treatment included three plants. Data are means (±SD) of three independent experiments. Significant differences were determined using Student’s t-test (*P<0.01, **P<0.001), compared with 22 °C in (A, B) and compared with the control in (E, F).