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. 2018 Feb 1;69(8):1967–1980. doi: 10.1093/jxb/ery029

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© The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — Comparison of copy number estimates obtained from qPCR assays and from low-coverage genomic data for the genes ppc_1P3 and ppc_1P6 in six A. semialata accessions. Copy numbers are expressed relative to the ppc_1P7 gene. Error bars are SEs from 2–3 technical replicates for qPCR estimates, and non-parametric error estimates from the GC correction resampling procedure for the genomic estimates of copy number. Dashed lines on the genomic estimates indicate confidence intervals for single-copy genes. The upper panel indicates the correlation between qPCR estimates (mean value of both pairs of primers) and genomic estimates of copy number for ppc_1P3 and ppc_1P6, with the red line being the regression line and the dashed line the identity line.