Table 1. Exocytotic phenotypes of clones transformed with different transgenes.

Transgene	Host strain
	Wild-type	nd7-1
Uninjected control	+	–
pT4+	+	ND
pT4–	–	ND
pT4pro	–	ND
pT4ter	+	ND
pND7+	+	+
pND7–	–	–
pND7pro	–	+ → –a
pND7ter	+	+

This table summarizes the effects of injection of different transgenes on the exocytotic phenotype of wild-type and nd7-1 mutant cells. The transgene constructs are given in Figure 1. Results were scored for 5–12 independent clones. In all cases where injection of transgenes did not affect the exocytotic phenotype of the host strain, clones maintaining >30 copies/haploid genome of the transgene, a value above the threshold necessary to obtain gene silencing, were selected for molecular analyses. ND, not done.

^aFunctional complementation is observed 24 h after injection but is then superceded by a silenced phenotype starting 48 h after injection.