Fig. 7.

(A) Gene expression changes of CsTRY in CsMYB6-overexpressing cucumber plants. Asterisks indicate statistically significant differences according to Student’s t-test (**P<0.01) WT, wild type. (B) Transient transcriptional activity assay showing the repression of the CsTRY promoter by CsMYB6. Asterisks indicate statistically significant differences according to Student’s t-test (**P<0.01). (C) Transactivation activity of CsMYB6 to the three MBS motifs in the promoter of CsTRY in yeast. (D) An electrophoretic mobility shift assay was used to analyze the interaction of GST-CsMYB6 and the three MBS motifs in the promoter of CsTRY. Purified GST-CsMYB6 protein samples (3 µg) were incubated with 25 pM of the biotin-labeled WT probe. Non-labeled probes at 50-fold concentrations were added for the competition test.