Fig. 3.

Positional cloning of DYE1. (A) Fine-mapping of DYE1. Analysis of the F₂ population and its progeny revealed that DYE1 is located between the markers SNP-3_RC and dCAPS-5 on Chromosome 8. The open circles indicate makers tightly linked with DYE1. dye1-1 and dye1-2 have single-nucleotide substitutions causing amino acid substitution in Lhca4. (B) Complementation of dye1-1 by a Lhca4 genomic clone. The chlorophyll contents of the flag leaves at heading are shown for wild-type Nipponbare, dye1-1, and the complementation transgenic line. Data are means and SE (n=4). **P<0.01; n.s., not significant (Student’s t-test).