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. 2018 Jun 26;7:e34135. doi: 10.7554/eLife.34135

Figure 2. Generation and characterization of lateral amygdala (LA)-selective glucocorticoid receptor (GR) knockout (LAGRKO) mice.

(A) The LAGRKO mouse line (GRflox/flox, Grp-iCre+/-) was established by crossing floxed GR (GRflox/flox) and Grp-iCre mice. Double immunofluorescence staining of GR (green, left panels) and NeuN (magenta, middle panels) in coronal brain sections from floxed GR and LAGRKO mice. The overlap of green and magenta signals (white, right panels) indicates the expression of GR in LA neurons in floxed GR mice (upper), which was apparently reduced in LAGRKO mice (lower). Magnified images of the boxed areas are shown in the insets. LA, lateral nucleus of the amygdala; BA, basal nucleus of the amygdala; CeA, central nucleus of the amygdala. (B) Quantification of GR+ and NeuN+ cells in the LA of floxed GR and LAGRKO mice (n = 9 sections from three mice). Data are presented as mean ± S.E.M. **p<0.001. (C) Expression of GR in the cerebral cortex and hippocampus.

Figure 2—source data 1. Raw data for generating Figure 2B.
elife-34135-fig2-data1.xlsx (10KB, xlsx)
DOI: 10.7554/eLife.34135.006

Figure 2.

Figure 2—figure supplement 1. Expression of GR in the LA and cerebral cortex of floxed GR and LAGRKO mice.

Figure 2—figure supplement 1.

(A) Double immunofluorescence staining of GR (green, left panels) and NeuN (magenta, middle panels) in the LA. The overlap of green and magenta signals (white, right panels) indicates the expression of GR in neurons. Scale bar = 50 μm. (B) Expression of GR in the cerebral cortex. Scale bar = 100 μm.