Figure 4. c-opsin1 knockout larvae lack UV-violet responses.

(A) Schematic of the Platynereis c-opsin1 gene and the c-opsin1^Δ8/Δ8 mutation. (B) Background GCaMP6s signal during calcium imaging in wild type and c-opsin1^Δ8/Δ8 mutant larvae. (C) Calcium responses to 70 s 405 nm stimulation in wild type and mutant larvae (n > 28 individual larvae for both genotypes). The stimulus light was switched on at 0 s. (D) Vertical swimming in wild type and mutant trochophore larvae stimulated with blue (480 nm) and UV (395 nm) light from above (n = 19 for both genotypes). The data are shown in 30 s bins. P-values: ***<0.0001; T-test with Sidak-Bonferroni correction.