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. 2018 May 31;7:e35122. doi: 10.7554/eLife.35122

Figure 6. Nitrogen assimilation pathways in Chlorella A99.

(A) Schematic diagram of the nitrogen assimilation pathway in plants showing the function of nitrate transporters NRT1 (peptides/nitrate transporter) and NRT2 (nitrate/nitrite transporter), nitrate assimilation-related components NAR1 and NAR2, nitrate reductase NR, nitrite reductase NiR, ammonium transporter AMT, glutamate synthetase GOGAT and glutamine synthetase GS. Genes shown in red boxes (NRT2, NAR2 and NiR) were not found in the Chlorella sp. A99 genome. (B) Table showing the number of nitrogen assimilation genes in Chlorella sp. A99 (A99), Chlorella variabilis NC64A (NC64A), Coccomyxa subellipsoidea C169 (C169), Volvox carteri f. nagariensis (Vc), Chlamydomonas reinhardtii (Cr), Ostreococcus tauri (Ot) and Micromonas pusilla (Mp). (C) Gene clusters of nitrate assimilation genes around the shared NR genes (blue) in the genomes of NC64A, C169 and A99. Red boxes show nitrate assimilation genes absent in A99 and gray boxes depict other genes. Numbers below the boxes are JGI protein IDs of NC64A and C169. Numbers below the genes of A99 are JGI protein IDs of the best hit genes in NC64A and C169 and their gene name.

Figure 6.

Figure 6—figure supplement 1. PCR of nitrate assimilation genes.

Figure 6—figure supplement 1.

PCR amplification of genomic DNA corresponding to the genes NRT2, NiR and NR (positive control) was performed in Chlorella sp. A99 (A99), Chlorella variabilis NC64A (NC64A), Coccomyxa subellipsoidea C169 (C169) and Chlamydomonas reinhardtii (Cr).