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. 2018 May 31;7:e35122. doi: 10.7554/eLife.35122

Figure 7. Growth of green algae in presence of various nitrogen sources.

The growth rate of Chlorella A99 (A99), Chlorella variabilis NC64A (NC64A) and Coccomyxa subellipsoidea C-169 (C169) by in vitro culture was assessed for different nitrogen sources with casamino acids (blue), glutamine (orange), ammonium (gray) and nitrate (yellow). Mean number of algae per ml were determined at 4, 8, 12 days after inoculation with 106 cell/ml. Error bars indicate standard deviation.

Figure 7.

Figure 7—figure supplement 1. PCR of 18S rRNA genes in cultured algae.

Figure 7—figure supplement 1.

PCR amplification of genomic DNA of the 18S rRNA gene was performed in Chlorella A99 shortly after isolation from H. viridissima A99 (Isolated A99), cultured in medium containing glutamine (Glu) and in medium with casamino acids for 12 days, with cultured NC64A and C169 added for comparison.