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. 2018 Jun 5;7:e37231. doi: 10.7554/eLife.37231

Figure 5. Demethylation of H3K4me2-modified nucleosomes by LHC.

(A) Demethylation of 100 nM H3K4me2 modified nucleosomes with either 146 or 185 bp nucleosomal 601 DNA by 400 nM LHC. (B) Corin inhibits the demethylation of the 185 bp H3K4me2 modified nucleosome substrate. These assays were performed three times (n = 3) in A and twice (n = 2) in B on separate occasions.

Figure 5.

Figure 5—figure supplement 1. Demethylation kinetics of H3K4me2 nucleosomes (100 nM) by LHC (400 nM) quantified by western blots.

Figure 5—figure supplement 1.

(A) The H3K4me2 nucleosome with 185 bp dsDNA was slowly demethylated by the CoREST complex while nucleosome demethylation with with 146 bp dsDNA was below the level of detection. (B) The LSD1 and HDAC1 dual inhibitor Corin (20 μM) inhibited the demethylation of the 185 bp nucleosome substrate; (n = 3 for A and n = 2 for B); error bars represent S.E.M.