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. 2001 Nov 1;29(21):4472–4481. doi: 10.1093/nar/29.21.4472

Figure 1.

Figure 1

Overexpression of PHR2 in Chlamydomonas. (A) Western blot analysis of whole cell lysates from cw15arg7 and G7 (PHR1) cells before and after heat shock. The G7 (PHR1) strain was derived by transformation of cw15arg7 with the overexpression construct HSP PHR2 Myc-His. Equal amounts of protein were separated by SDS–PAGE, transferred to PVDF and blotted with anti-Myc antibody. The position of the 75 kDa marker is indicated on the left. (B) Southern blot analysis of genomic DNA from cw15arg7 and G7 (PHR1) cells. DNA was digested with BglII and PstI and hybridized with radiolabeled PHR2 5′ PE probe. The endogenous PHR2 gene is indicated on the right.