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. 2001 Nov 1;29(21):4472–4481. doi: 10.1093/nar/29.21.4472

Figure 2.

Figure 2

Overexpressed PHR2 Myc-His binds UV-irradiated DNA. (A) Aliquots of 10 µg ammonium sulfate protein extract from heat shocked and non-heat shocked cw15arg7 and G7 (PHR1) cells were incubated in the dark at 25°C for 30 min with radioactively labeled DNA probe that had or had not been UV-irradiated as indicated. The mixtures were then run on a 5% non-denaturing polyacrylamide gel and visualized by autoradiography. (B) Western blot analysis of ammonium sulfate protein extracts from cw15arg7 and G7 (PHR1) cells before (lanes 1 and 3) and after (lanes 2 and 4) heat shock. Equal amounts of protein were separated by SDS–PAGE, transferred to PVDF and blotted with anti-Myc antibody. The position of the 75 kDa marker is indicated on the left.