Correlation of the serum levels of 24S-HC with the expression of spectrin breakdown products (SBDP) at 6hr and 24hr after neonatal HI. A. Protein expression of SBDP145/150KD and SBDP120KD in the cortices was measured by western blotting at the indicated time points and presented as the OD ratio to β-actin and normalized to the values of sham 0hr (for SBDP145/150), or an internal control (IC, for SBDP120). For SBDP145/150KD, sham vs. HI, *p=0.0094 at 6hr, *p=0.0013 at 24hr. For SBDP120KD, sham vs. HI, *p=0.036 at 6hr, *p=0.0087 at 24hr. (n=4–6 for sham animals, n=6–15 for HI animals from 6–72hr). B. Correlation of the serum levels of 24S-HC with the expression of SBDP145/150KD at 6hr (top), at 24hr (middle), and with the expression of SBDP120KD at 24hr (bottom). Sample number, R2 and p values are shown in the graphs.