Table 1.
Possible origins of isoallergens, variants and other types of allergen heterogeneity.
Type of heterogeneity | Origin | Characteristics | Known examples in the Allergen Nomenclature database |
---|---|---|---|
Dispersed amino acid sequence variability. Named as isoallergens or variants, depending on level of sequence divergence. | Allelic | One isoallergen/variant per haplotype.a | Der f 2; Bet v 1 variants are in part allelic |
Differential IgE antibody recognition of isoallergens may occur but is less likely for variants. | |||
Multigene family | Multiple isoallergens/variants, encoded by different genes per haplotype, may be present per organism.a | Bet v 1 | |
Differential IgE antibody recognition of isoallergens may occur but is less likely for variants. | Ara h 2 | ||
Size and/or charge distribution | Posttranslational modifications such as addition of carbohydrate, phosphate groups, hydroxy-proline, C-terminal proteolytic cleavage or other moietiesb | Each individual proteins may contain multiple postranslationally modified allergen forms. | No examples |
Differential IgE antibody recognition of glycoforms may occur. | |||
Chemical composition | Chemical modification of the allergenb | Modification of the allergen during airborne transport (for aeroallergens), storage and processing (for food allergens), or preparation of extracts, e.g. oxidization, reactions with polyphenols, Maillard reaction during roasting of foods. | No examples |
Block differences: amino acid sequence differences in segment/s of the protein. | Different protein cleavage | Each individual contains multiple differentially cleaved forms. | Hev b 6.02 and Hev b 6.03 |
IgE antibody recognition may be affected depending on epitope distribution. | |||
Alternative mRNA splicing | None yet identified for allergens. | No examples | |
Modular differences: Different subunit (entire gene product) composition. | Alternative subunit composition | Each individual contains multiple allergen forms, depending on subunit combinations. | Gly m 5, Gly m 6 |
IgE antibody recognition may be affected depending on epitope distribution. |
Polyploidy (more than two haplotypes) will further increase the number of genes (each of which can harbor different allelic isoallergens/variants).
This kind of modification does not lead to different isoallergens or variants.