Fig. 3.

DDLPS cells reconstituted with miR-133a show shifted mitochondrial function linked to energy balance. a Mitostress Test was performed in DDLPS cell lines 224B, 863, and 246. b OCR values shown after injection of FCCP relative to baseline OCR measurement (n = 3). *p < 0.05. c Glycostress Test was performed in DDLPS cell line 246. d Basal glycolysis was measured by baseline ECAR subtracted by non-glucose-derived ECAR (ECAR values after injection of 2-deoxyglucose). e Real time quantitative PCR analysis was performed with primers against the MFN2 gene. Primers to B2M were used to normalize the samples. Error bars represent standard error of mean for the replicate values. D 2-deoxy-d-glucose, ECAR extracellular acidification rate, F carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone), G glucose, OCR oxygen consumption rate, O oligomycin, R rotenone