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ALK在不同亚型肺腺癌中的表达情况及FISH验证。A：ALK在乳头型肺腺癌肿瘤细胞胞浆内高表达（×200）；B：ALK在实体型肺腺癌肿瘤细胞胞浆内高表达（×200）；C：ALK在腺泡型（筛状型）肺腺癌肿瘤细胞胞浆内高表达（×200）；D：ALK IHC阳性腺泡型肺腺癌FISH检测示肿瘤细胞核内红、绿信号分离或出现额外的红色信号（箭头示），提示ALK基因断裂或重组，即阳性。

Expression of ALK in 4 EGFR mutated lung adenocarcinomas with different histologic subtypes and the confirmation by FISH. A: High expression of ALK in cytoplasm of papillary predominant adenocarcinoma (×200); B: High expression of ALK in cytoplasm of solid predominant adenocarcinoma (×200); C: High expression of ALK in cytoplasm of acinar (cribriform) predominant adenocarcinoma (×200); D: In ALK IHC positive acinar predominant adenocarcinoma, the results of a break-apart FISH assay of tumor cells with separation of red and green probe signals or extra red probe signals (arrows) indicates a break of ALK or maybe chromosomal rearrangement involving ALK. The FISH result is positive.