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. 2018 Jun 27;38(3):BSR20180568. doi: 10.1042/BSR20180568

Figure 1. The conserved phenylalanine of motif IV is required for both ATP and DNA binding in the presence of the other ligand.

Figure 1

(A) Sequence alignment of the motif IV present in the SWI2/SNF2 proteins. Alignment was carried out using Clustal W program [41], and some minor editing was done manually. Highlighted box represents the conserved Motif IV, of which conserved phenylalanine residue was chosen for the mutation. (B) Comparison of ATPase activity of wild-type ADAAD, F507A, and F507W. (C) Comparison of kinetic data of F507W and wild-type ADAAD. (D) Comparison of CD spectra of F507A with the wild-type ADAAD in the absence of ligands. (E) Comparison of CD spectra of F507A with the wild-type ADAAD in the presence of ligands. (F) Comparison of CD spectra of F507W with wild-type ADAAD in the absence of ligands. (G) Comparison of CD spectra of F507W with wild-type ADAAD in the presence of ligands.