Supplementary Figure 7.

(A) Diagrammatic representation depicting inter-lobe communication in eIF4 [1] (B) In ADAAD the covariance in the positions of aspartate and histidine is observed leading us to ask whether a possible salt bridge exists between motif II and motif VI. (C) To test the hypothesis, the aspartate (D591) present in motif VI was mutated to histidine which resulted in abrogation of ATPase activity. (D) The ATPase activity was not restored when the histidine (H329) present in motif II was mutated to aspartate.

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